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cytotoxicity and genotoxicity of Solanum tuberosum leaves using Brine shrimp lethality bioassay and Allium cepa chromosomal.
By JOHN ADEWALE
This study aims to determine the cytotoxicity and genotoxicity of Solanum tuberosum leaves using Brine shrimp lethality bioassay and Allium cepa chromosomal aberration assay. Specifically, this study aims to determine the following: 1. Determine the percent mortality based on the dead nauplii after exposure to the leaf extract. 2. Determine the percent cytotoxicity of the leaf extracts based on the percent mortality of dead nauplii. 3. Determine the median lethal concentration (LC50) of the leaf extract. 4. Determine the genotoxicity activity of the plant extract based on the following: a. percent root growth after exposure. b. percent chromosomal aberration as indicated by the presence of bridges, fragments, laggards or vagrants. c. mitotic index after exposure. 80 PAGES ABOVE | CHAPTER 1-5